. Step 2: Launch SoftMax Pro Software. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode SpectraMax® Microplate Reader User Manual Download Page. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. . Uncontrolled Copy for Training and Reference Purposes Only . Molecular Devices' microplate readers. Supersedes 1. The automated ELISA workflow has two positive outcomes. Measure more accurately. This document is provided to customers who have purchased Molecular Devices, Inc. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. SoftMax Pro 7. Plus 384. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Supplier: Molecular Devices. 40266 Email: drugdiscoverytech@lifetech. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. AXON Patch-Clamp. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The instrument supports the following read modes:. Read times. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Molecular Devices. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. A). 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Molecular Devices, LLC. Fluorescence Microplate Reader Comparison Chart. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. Plate chamber design of the SpectraMax category 1 microplate readers. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Imaging System Giles Scientific Biomic V3 Automatic Plate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Examples include ELISAs for quantitation of cytokines and protein concentration determination using the Bradford assay. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. IMAP assays are based on binding of. Instrument settings can be saved as a protocol file. See More Microplate Reader / Microtiter Plate Reader. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. 2. 7°C and the bottom to 36. When connecting for the first time to either the SpectraMax L reader or StakMax system. To update the driver. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. , June 1, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of FLIPRTETRA the first system that enables researchers to significantly increase throughput of cell-based assays by measuring 1536 kinetic measurements simultaneously. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. PURPOSE. Integrated stacker option. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 9. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. 0 . Additional high-quality filters to. 340 PC384. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 2. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Detector (s) PMT and/or photodiode. One plate carrier adapter is provided with the instrument. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. DOCUMENTATION. View the Molecular Devices website. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0200-6262. 303 unable to cal dark current. together with a Molecular Devices plate. The sample is typically placed in a cuvette through which light is sent horizontally. In . Supersedes 1. One plate carrier adapter is provided with the instrument. The adapter is required for optimum performance with standard 96-well and 384-. Your applications, your modes, your choice. Recombinant protein expression Nucleocapsid. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. tools to expand the boundaries of their research. , attenuation mode = out. Methods. Lamp source is a Xenon flash lamp (1 Joule/flash). This process should take about 3 - 5 minutes. Published Dec 16, 2020 | Updated Dec 16, 2020. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Feb 22, 2023Used. Lift up the lid over the chamber to insert or remove a cuvette. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. No accessories are needed and SoftMax® Pro Software. 1. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. 1 sec. 3. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. For biochemical assays, the washer removes unbound material and unreacted reagents. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. The presence of VCAM-1 indicates a positive inflammatory response. 2. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Double-click the unknown device with the yellow warning icon. 2. Latest Citations: For a complete list, please click here. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. These robust readers have been placed in labs from. July 17, 2023. 2. 40266 Email: drugdiscoverytech@lifetech. 0 . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. SpectraDrop High-Throughput Screening (HTS) Kit. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 00. Plate Formats 6 to 1536 wells. Laboratory automation workflows can help. Company Molecular Devices, LLC. W×D×H. Experimental details and customization of the group table. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. visible absorbance, fluorescence. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Wait 20 seconds. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. A preconfigured. 1. A. Image courtesy of Molecular Devices. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Plate reader. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The lower limit of detection (LLD) was defined as the level of. Tests every subsystem of the reader or . This process should take about 3 - 5 minutes. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Also refer to the Multimode Reader Comparison Chart Features. We would like to show you a description here but the site won’t allow us. With optimized reagents, option to operate the. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 18 kg. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. +1 800-635-5577. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Download PDF versions of the SpectraMax Microplate Reader User Guides. Category 2 and 3 microplate readers use gradient heating as well. 304 signal level saturation. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. M5. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of. 0 . Shop now. Plate stacker and robot integration The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within minutes and begin reading microplates with seven mouse clicks. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). The plate is set up for reading and images are acquired according to specified parameters. 312 gain calibration failed. Load up the plates that you need to run and walk. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. SoftMax Pro Software. Explore our fully-integrated,. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. SpectraMax Microplate Readers. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. (SPR) were monitored by Clone Select Imager (CSI, Molecular Devices, San Jose, CA, USA) and by ELISA assay according to the protocol in Brezinksy etFLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Versa. (Figure 1). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. These lab workhorses are used in drug discovery. Plate section without PathCheck applied. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. The plate was then re-read on the plate reader (Read B). This reader's dual monochromators for. Plate reader instructions Computer. ) Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 32 was diluted 10-, 100-,. FlexStation® 3 Multi-Mode Microplate Reader. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Page 1 of 8 . FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The cuvette must be in place when you read the plate. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. EMax. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. United States Phone: 800-635-5577 408-747-1700. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. 5 µL of each sample was pipetted into a 96-well. and the assembly was incubated for 5 minutes in the dark prior to reading the plate. Fluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. 3860 N First Street San Jose, CA 95134. 13. book Page 1 Tuesday, July 6, 2010 2:08 PM Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. A standard spectrophotometer measures the absorbance of one sample at a time. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Visible absorbance -. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Supplier: Molecular Devices Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength. Also see: SoftMax® Pro Microplate Software User Manual Download Page. Supplier: Molecular Devices. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Jun 01, 2004. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Temperature control and shaking come standard. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. Bandwidth for both excitation and emission is 9 nm. Plus 384. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. . Endpoint. Temperature Control 4°C above ambient to 45°C. Molecular Devices products are warranted to meet the stated specifications. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Read height should be optimized for Vmax® plate reader specifications. #SpectraMax L Config). This offer includes SpectraMax i3x, SpectraMax. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. SoftMax® Pro 7. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. with the kit reagent and transferred to the plate reader for evaluation at the same time. 305 reference level saturation. 25 to 3. The whole plate can be detect in a single run with ease and flexible. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 1. Step. plate type, read area and number of images per well, plus specify positive/negative wells. The computer controlling the plate reader runs Windows XP. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. begin reading microplates with seven mouse clicks. The plate reader optics are tunable for absorbance,. Clone Screening. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. 1. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. D. Molecular Devices technical support is available by phone and e-mail. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. To contact technical support by phone in the U. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. For SpectraMax ABS Plus reader, r 2 = 0. 5: Schematic of the back panel of a reader. M2. With optimized reagents, option to operate the. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. ” So, acquisition and analysis come from one software system. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Superior optics Microplate Reader Shelf. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Published Dec 16, 2020 | Updated May 19, 2023. We would like to show you a description here but the site won’t allow us. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. The plate is set up for reading and images are acquired according to specified parameters. 2. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Select the Driver tab and click Update Driver. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 1) is a fluorescence plate reader that can measure intracellular Ca 2+ concentration using both single- and dual-wavelength fluorescent probes. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Avoid losing high signal. 4 °C above ambient to 45 °C. 00. 5. 9000-0756. Some familiarity with Windows should be sufficient to use it. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 6 kg (30 lbs. The Synergy H4 is a convenient benchtop microplate reader that offers robust. The direct transfer capability reduces We would like to show you a description here but the site won’t allow us. Step 1: Turn on your microplate reader. Every step is optimized to simplify analysis and reporting to support our microplate readers. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. The reader measures both flat and round 96-well microplates and. Microplate Reader, Multimode. Catalog Number i3x. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. 8×15×8. Molecular Devices' microplate readers. One plate carrier adapter is provided with the instrument. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. absorbance plate readers. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. integration time per well, depending on firmware version. Timothy Bolus, MPM, MBA, Compliance Program Manager. If the computer is connected to the Internet: Select Search Automatically for. 1. With the discontinuous kinetics feature in SoftMax Pro Software. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The guide also covers the software features, the specifications, and the accessories of the systems. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. Other features and benefits include: Eliminate changing out filters. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Integrated to. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. (a) Chemical structures of 1 (HADP) and its analogues 2–4. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. Test up to 3. Lab automation for high-throughput plate-based assays. S. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. After reading the ELISA plate on both readers, each standard curve was nearly identical. Microplate Stacker and Washer. . Instrument settings can be saved as a protocol file. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. This can increase throughput and efficiency, thus saving time and space in the lab. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. The plate reader. 997 (n = 3). Figure 1. The adapter is required for optimum performa nce with standard 96-well and 384-. 3860 N First Street San Jose,CA 95134 USA +1 408. Next generation SpectraMax® absorbance microplate readers. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. View the Molecular Devices website. Read plate standard and mix. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required.